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Production of HIV-based virus-like particles

Virus engineering

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Complex problems often require elegant solutions. Virus-like particles have potential as non-infectious vaccines & as delivery vehicles for heterologous antigens & therapeutic proteins. The toxicity of HIV glycoprotein expression in mammalian cells limited production of recombinant particles. Re-engineering the HIV genome enabled efficient production of budded VLPs.

Structure of a minimal HIV construct expressing Gag, Tat & Rev proteins & containing regulatory elements tar & rre

Example:

Production of HIV-based Virus-like particles

 

Problem:

The HIV glycoprotein is "toxic" & cannot be overexpressed in mammalian cells

 

Solution:

Re-engineer the HIV genome to express Gag within a natural Tat/tar, Rev/rre regulatory & RNA splicing context. Electron micrographs of transfected Vero cells demonstrated expression of budding virus-like particles

 

Patent Link:

Czaplewski LG PROTEINACEOUS PARTICLES CONTAINING RETROVIRAL ELEMENT

HIV