Virus engineering

Production of HIV-based virus-like particles

Complex problems often require elegant solutions. Virus-like particles have potential as non-infectious vaccines & as delivery vehicles for heterologous antigens & therapeutic proteins. The toxicity of HIV glycoprotein expression in mammalian cells limited production of recombinant particles. Re-engineering the HIV genome enabled efficient production of budded VLPs.

Example:
Production of HIV-based Virus-like particles

Problem:
The HIV glycoprotein is "toxic" & cannot be overexpressed in mammalian cells

Solution:
Re-engineer the HIV genome to express Gag within a natural Tat/tar, Rev/rre regulatory & RNA splicing context. Electron micrographs of transfected Vero cells demonstrated expression of budding virus-like particles

Patent Link:
Czaplewski LG PROTEINACEOUS PARTICLES CONTAINING RETROVIRAL ELEMENT

HIV

Structure of a minimal HIV construct expressing Gag, Tat & Rev proteins & containing regulatory elements tar & rre

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